Impact of Pasteurization on Ascorbic Acid in Orange Juice (Overview)
Abstract
Loss in Ascorbic Acid contents of orange juice heated under different situations and pH was researched. In this experiment, Navanila oranges from Spain were squeezed and filtered by using a vacuum pump to make orange juice samples. These samples were extracted and make up with 0.1% Formic Acid in HPLC water, HPLC water and phosphate buffer solution (pH=6). Pasteurization was achieved at 60, 65 and 70℃ over 15, 25 and 35min period by using the water bath method and cooled to room temperature immediately. After that, Ascorbic Acid contents were analyzed by HPLC and 0.1% Formic Acid in HPLC water, HPLC water and phosphate buffer solution (pH=6) were used as mobile phases separately for their solutions. HPLC standard samples of Ascorbic Acid were prepared which means 0.1g Ascorbic Acid was weighted and then diluted into 50-250mg/ L solutions to make calibration lines for three solutions. The wavelength of Ascorbic Acid was 245nm but it changed to 296nm when samples in phosphate buffer solution (pH=6). Results showed that Ascorbic Acid concentration is more at 60℃ at 15min and there was a total decline trend with the increase of time and temperature. After statistics analysis, it has a significant affect (P<0.5) related to Ascorbic Acid contents with temperature and pH.References
Allison Cooke. (2017) ‘Dietary Food-Additive Phosphate and Human Health Outcomes.’ Journal of Food Science and Food Safety. 16(5). 1541–4337.
Anitra C. Carr, and Jens Lykkesfeldt. (2017) Vitamin C in Health and Disease.MDPI St.Alban-Anlage 66 Switzerland: Published online Nutrients.
Isam Eldin H. Elgailani, Mohamed A. M. Gad-Elkareem, Elnoor A. A. Noh, Omer E. A. Adam, Ahmed M. A. Alghamdi (2017) ‘Comparison of Two Methods for The Determination of Vitamin C (Ascorbic Acid) in Some Fruits.’Journal of Chemistry.2(1). pp. 1–7.
Lanfranco S. Conte, Sabrina Moret, and Giorgia Purcaro. (2010) Handbook of HPLC 2rd. Chromatographic Science Series volume11. Edited by Danilo Corradini. New York: CRC Press.
L. Cinquanta, D. Albanese, G. Cuccurullo, and M. Di Matteo. (2010) ‘Effect on Orange Juice of Batch Pasteurization in an Improved Pilot-Scale Microwave Oven,’Journal of Food Science, 75(1750). E46–E50.
Luiz Trugo and Paul M. Finglas. (2003) The Encyclopeida of Food Sciences and Nutrition, Second Edition. Food Technology and Nutrition. 10(6000). 7–17. Elsevier Science Ltd.: Academic Press.
Maria I. Gil, Encarna Aguayo, and Adel A. Kader. (2006) ‘Quality Changes and Anutrient Retention in Fresh-Cut versus Whole Fruit during Storage.’Journal of Agriculture Food Chemistry. 54(12). 4284–4296.
Nicholas N. DePhillipo, Zachary S. Aman, Mitchell I. Kennedy, J. P. Begley, Gilbert Moatshe, and Robert F. LaPrade. (2018) ‘Efficacy ofVitaminCSupplementation onCollagen Synthesis and Oxidative StressAfter MusculoskeletalInjuries:ASystematic Review.’ Orthop J Sports Med. 6(10). pp. 3–6.
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