Vol. 4 No. 1 (2022)

Open Access

Original Research Article

Article ID: 2653

Correlation Analysis of Patients with Adverse Reactions Caused by Postoperative Morphine Analgesia

by Guolei Du, Lijuan Wan, Mengqian Zhang, Bingzheng Luan

Reports in Clinical Studies and Medicine, Vol.4, No.1, 2022; 46 Views, 5 PDF Downloads

  I n the current medical field of clinical surgery, we often have to contact a large number of patients with severe fractures caused by serious accidental injuries. Generally, these trauma patients often need to be treated by repair surgery. Postoperative patients usually have a certain degree of local pain. In order to alleviate the physiological pain of postoperative patients, And can prevent the braking caused by the aggravation of local pain of the injured patients. The use of analgesic pump for postoperative analgesia has become a common method after surgery and has been widely used in clinic. Good postoperative analgesia can inhibit the body's stress response and is conducive to restoring the coordination and stability of local breathing and circulation of the patients after operation, Reduce the local pain symptoms of some patients and effectively reduce their postoperative related complications, and indirectly promote the recovery of patients with pain. For the application of analgesic pump for pain relief, there are often a variety of auxiliary ways and preparation methods of analgesic drugs such as arteriovenous and epidural circulation, which should be selected and configured according to the physical conditions and medication conditions of patients with pain, such as ns100ml Morphine 5mg, bupivacaine 75mg, etc., of which morphine is often used as a common drug component for pain relief, but some special patients often have a series of dependent reactions after using morphine for pain relief. In view of this situation, combined with the physical conditions and adverse reactions of some special patients, this paper analyzes the possible adverse effects on patients with special constitution when using morphine for pain relief after clinical operation and relevant research.

Open Access

Original Research Article

Article ID: 2916

Research Progress of Neural Stem Cell Transplantation Combined with Biological Scaffold in the Treatment of Spinal Cord Injury

by Tao Li, Tanlong Wang

Reports in Clinical Studies and Medicine, Vol.4, No.1, 2022; 37 Views, 3 PDF Downloads

SCI is a kind of nerve injury disease caused by direct or indirect factors. The result is the damage of motor mechanism and the partial or total loss of sensory function below the injury site. The researchers found that planting neural stem cells in a biological scaffold and then colonizing the site of SCI greatly improved the survival rate of stem cells and promoted the repair of injury. However, different biomaterials have different differences. In order to better promote the recovery of function after SCI, it is important to select an appropriate scaffold combination.

Open Access

Original Research Article

Article ID: 2915

Research Review on Effects of Exercise on Neuroglial Cells after Central Nervous System Diseases

by Mengqi Ma, Yu Wu

Reports in Clinical Studies and Medicine, Vol.4, No.1, 2022; 59 Views, 6 PDF Downloads

Astrocyte and microglia are two types of neuroglia that are relatively widely distributed and functionally important in the mammalian central nervous system. These two cells play an important role in CNS diseases. The mechanism of the effect of exercise on various CNS diseases is still unclear. In this paper, the effect of exercise on neuroglial cells after CNS diseases is investigated in depth from the function and structure of glial cells.

Open Access

Original Research Article

Article ID: 3175

Extracorporeal Shock-Wave Therapy in the Experimental Rat Osteoarthritis with Osteoporosis

by Qianyuan Wang, Haijun Liu, Kui Chen, Gengmei Xing, Gengyan Xing

Reports in Clinical Studies and Medicine, Vol.4, No.1, 2022; 100 Views, 6 PDF Downloads

Purpose: Ustilizing the osteoarthritis(OA) model rats with osteoporosis(OP) evaluate and anticipate the effect of extracorporeal shockwave therapy (ESWT) in various aspects to coexisting of OA and OP in elderly women. Methods:  Thirty-six female Sprague-Dawley rats were selected to make model of OPOA through surgery. Firstly, OP model were marked by the surgery of OVX, and were selected through micro-CT. Then, these OP model rats were treated by the surgery of ACL+MM. Finally, OPOA models were comfirmed by the hologiexamination. These model rats were divided into OPOA and OPOA + ESWT (n=12/group). Rats in the sham rats (n=12) were randomly selection from the group that were without treated by surgery. After ESWT, biomechanical analysis, micro-CT analysis, hologiexamination and immunohistochemical assay were utilized to evaluate the therapeutic effect. Mineralisation and alkaline phosphatase (ALP) in osteoblasts was analyzed to speculate the possible therapeutic mechanism of ESWT to OPOA.  Results:  Results of biomechanical analysis and hologiexamination show that ESWT could prevent further degeneration of articular cartilage. Micro-CT analysis and immunohistochemical assay demonstrate that the treatment could improve the microstructure of subchondral bone. Meanwhile, ESWT may improve the proliferation ability or activity of osteoblasts including in subchondral bone of OPOA rats in vivo . Conclusion:   Our research suggests that ESWT prevented cartilage damage progression in the development of OPOA through intervening to degradation of subchondral bone.

Open Access

Original Research Article

Article ID: 2899

Conflict and Balance Between Intellectual Property Protection of Pharmaceuticals and Public Health

by Ruyi Xiao

Reports in Clinical Studies and Medicine, Vol.4, No.1, 2022; 65 Views, 6 PDF Downloads

In December 2019, the emergence of unexplained pneumonia (later named COVID-19) caught people all over the world off guard. Until now, the epidemic situation is still not optimistic, and global health is seriously threatened. Prevention and control measures for COVID-19, accurately all infectious diseases, people are no longer strange. There are three ways, that is, to control the source of infection (isolation), cut off the transmission path (wearing masks), and protect the susceptible population (vaccines and specific drugs). [1 ]   A mong them, the last link - screening and developing new drugs and vaccines is the focus of the current efforts of medical institutions, pharmaceutical companies, and scientific research institutions. In January 2020, the news that Chinese researchers at the Wuhan Institute of Virology filed for a patent on the use of Remdesivir, an investigational drug from the US company Gilead, caused widespread controversy, because patent protection would limit people's access to vaccines and drugs. [2 ]  This also raises the question of how to balance the conflict between intellectual property protection for new pharmaceutical products and public health in the face of a global pandemic. This article mainly discusses three aspects, the fact of high cost, increasing complexities, and high rewarding of new pharmaceuticals, the status of strong protection in the pharmaceutical industry, and the negative public health impact of this strong protection. Although WTO members and some countries have responded accordingly to the consequences, the effect has been limited. This article will use this three-aspect analysis to in turn provide some information to increase the availability of vaccines and medicines for COVID-19 and other diseases.

Open Access

Original Research Article

Article ID: 2902

Efficient Screening of nNOS-PSD95 Uncoupling Agents Based on Radiometric Fluorescent Molecularly Imprinted Sensors

by Xicheng Yang, Yankun Gao, Hongjuan Zhang, Hongliang Xin

Reports in Clinical Studies and Medicine, Vol.4, No.1, 2022; 39 Views, 2 PDF Downloads

Novel and efficient ratiometric fluorescent molecularly imprinted sensors (RFMIS) based on epitopes were developed, which can be used for the sensitive detection of neuronal nitric oxide synthase in the screening of neuronal nitric oxide synthase-postsynaptic density95 (nNOS-PSD95) coupling inhibitors. Under appropriate conditions, the fluorescence of the carbon dots quenched with the increasing concentration of nNOS1-133, while the fluorescence of the quantum dots remained unchanged. The fluorescence ratio had a good linearity in the concentration range of 0-500 ng mL-1 for nNOS1-133 and the determination limit was 0.14 ng mL-1. Using the classical nNOS-PSD95 coupling inhibitor (ZL006) as a control, the RFMIS were used as the detector to detect the free nNOS released by Gnetol and 2,3,5,4ʹ-tetrahydroxystilbene-2-O-β-Dglucoside from natural medicine after inhibition of nNOS-PSD95. The results have shown that the uncoupling efficiencies was consistent with co-immunoprecipitation experiments. The study provides a new idea and a new way for efficient screening of nature nNOS-PSD95 coupling inhibitors from natural medicine with the advantages of high efficiency, sensitivity and traceability.

Open Access

Original Research Article

Article ID: 2897

Analysis of HER2 Gene Amplification and Certain Prognostic Factors in Breast Cancer

by Xulong Zhu, Binliang Huo, Jianhui Li, Shuhan Wu, Yangmeng Feng

Reports in Clinical Studies and Medicine, Vol.4, No.1, 2022; 61 Views, 3 PDF Downloads

Objective: The HER2 gene amplification and certain prognostic factors in breast cancer were analyzed. Method: The gene amplification and protein expression of human epidermal growth factor receptor in 100 breast cancer tissues detected by FISH and IHC detection method in the hospital from January 2020 to December 2021 were analyzed. To analyze some breast cancer prognostic factors. Result: 0 is 8 cases of HER-2 protein breast cancer, (1+) is 11 cases, (2+) is 49 cases, (3+) is 32 cases. The HER2 gene was amplified in 49 cases, of which 23 cases showed red signals in clusters, and 26 cases showed red signals in dots. 51 cases of HER-2 gene were not amplified. There are differences in the detection results of FISH and IHC detection methods (P ＞ 0.05 ). ER, PR and polysomy of chromosome 17 are prognostic factors associated with HER2 gene amplification in certain breast cancers. (P ＜ 0.05 ) Conclusion: To analyze the HER2 gene amplification in breast cancer and targeted select FISH and IHC detection methods can improve the therapeutic effect and prognostic factor, which deserves clinical attention.