Vol 7 No 1 (2023)

Open Access

Original Research Article

Article ID: 833

Research status of relationship between schistosomiasis and colorectal cancer

by Xingguang Yang, Jiwu Yang, Xiangnong Cun, Pengju Zhao, Wei Cheng, Haibin Shi, Yi Li

General Surgery, Vol.7, No.1, 2023; 407 Views, 19 PDF (English) Downloads

In clinical practice, intestinal mucosal inflammation caused by schistosomiasis has great risks to lead to tumor proliferation and migration of cancer cells. Without timely and effective intervention, chronic schistosomiasis infection induced will further deteriorate into colorectal cancer. On the whole, schistosomiasis once caused serious infection to China. Patients suffering from colorectal cancer who have been infected with schistosomiasis can be diagnosed as schistosomiasis-related colorectal cancer if their preoperative intestinal tract has infection manifestations and postoperative pathology indicates that eggs are deposited near the focus. This paper explores the relationship between schistosomiasis and colorectal cancer by combining relevant data and literature at home and abroad.

Open Access

Original Research Article

Article ID: 852

Effect of chloroquine on the apoptosis of tongue squamous cell carcinoma SCC25 cells induced by bleomycin and nedaplatin

by Ruoyu Wang, Lan Yang

General Surgery, Vol.7, No.1, 2023; 409 Views, 8 PDF (English) Downloads

Objective:  To study the effect and mechanism of chloroquine-induced chemotherapy drugs bleomycin and nedaplatin on apoptosis of tongue squamous cell carcinoma SCC25 cells. Methods : The inhibitory effect of different drugs on the growth of SCC25 cells was screened by MTT assay. MTT, Hoechst33258 staining and FCM were used to observe the changes of morphology, cell cycle and apoptosis of SCC25 cells after different treatments. The quantitative analysis of chloroquine induced by Western blot The effect of chemotherapy drugs on the expression of Bax, Bcl-2 and NFkB apoptosis- related proteins in SCC25 cells. Results : The proliferation inhibition rate of bleomycin and nedaplatin SCC25 cells induced by chloroquine was significantly higher than that of bleomycin and nedaplatin alone (p<0.05); Hoechst33358 fluorescence staining and flow cytometry showed In the bleomycin group, the apoptotic rate of the chloroquine plus bleomycin group was significantly increased, and the apoptosis rate of the chloroquine + nedaplatin group was significantly higher than that of the nedaplatin group (p<0.05). The proportion of G0/G1 phase in the chloroquine + bleomycin group was higher than that in the bleomycin group (p<0.05), and the ratio of cells in the S phase to the G2/M phase was decreased; the same chloroquine + nedaplatin group G0/G1 The proportion of cells in the period was higher than that in the nedaplatin group (p<0.05), and the proportion of cells in the S phase and G2/M phase decreased. WB results showed that the expression levels of Bax and NFkB in chloroquine + bleomycin group were significantly higher than those in bleomycin group alone, and the expression level of Bcl-2 was lower than that in bleomycin group alone. Bax and NFkB in chloroquine + nedaplatin group The expression level was significantly higher than that of the nedaplatin group alone. The expression level of Bcl-2 was lower than that of the nedaplatin group alone, and the difference was significant (p<0.05). Conclusion:  Chloroquine induced bleomycin and nedaplatin can significantly inhibit the proliferation of tongue squamous cell carcinoma SCC25 cells, and its proapoptotic effect may be related to the activation of mitochondrial apoptosis-related proteins and nuclear transcription factors.

Open Access

Original Research Article

Article ID: 3352

Updates on Neuronavigation: Emerging tools for tumor resection

by Anjali Patel, Drashti Patel, Raja Al-Bahou, Rajvi Thakkar, Ivelina Kioutchoukova, Marco Foreman, Devon Foster, Brandon Lucke-Wold

General Surgery, Vol.7, No.1, 2023; 128 Views, 19 PDF (English) Downloads

Multiple studies have been conducted to properly elucidate the various tools available to help enhance the resection of tumor tissue, aneurysms, and arteriovenous malformations (AVM). Diffusion tensor imaging (DTI) tractography is useful in providing a map of the tumor borders, allowing the optimal preservation of function and structure of specific regions of the brain. During neurosurgery, especially craniotomies, the possibility of the brain shifting due to swelling or gravity is high. Thus, tools for intraoperative imaging such as high-frequency linear array ultrasound transducers and doppler ultrasonography are utilized for high resolution images and detecting frequency shifts. 4D-digital subtraction angiography (DSA) is another technique used to create spatial resolutions and 3D maps for aneurysms. These similar techniques can also be utilized to assess the integrity of white matter in AVM. By implementing effective evaluation strategies, healthcare professionals can make informed decisions regarding treatment options, preventive measures, and long-term care plans tailored to individual patients.

Open Access

Review Article

Article ID: 851

Circular RNA (circRNA) and YAP in tumor: A review

by Xu Li, Xiaodong Tian, Xiangdong Mu, Zhenzhen Liang, Xiaohui Xu, Haiyun Yu

General Surgery, Vol.7, No.1, 2023; 427 Views, 12 PDF (English) Downloads

Background:  Circular Ribonucleic Acid (circRNA) is a type of RNA that has been found in recent years and is a closed loop structure widely found in eukaryotic cells.  Present situation:  The circRNA is stable in nature, highly conserved and tissue and disease specific, and regulates target gene expression at the transcriptional or posttranscriptional level, which is related to the occurrence and development of tumors. YAP (Yes-associated protein) is the main effector of Hippo signaling pathway. It is found that YAP level is closely related to cell proliferation and apoptosis, tissue organ size control and tumorigenesis.  Aim:  to study the correlation between circRNA and YAP in tumors.