Vol. 10 No. 1 (2021)

Open Access

Original Research Articles

Article ID: 2108

Efficacy and Safety of Paclitaxel and Cisplatin in the Treatment of Advanced Ovarian Cancer by Different Means of Administration

by Datian Fu

Preventive Medicine Research, Vol.10, No.1, 2021; 52 Views, 4 PDF Downloads

In the treatment of advanced ovarian cancer, paclitaxel and cisplatin are administered in different ways. This paper discusses the efficacy and safety of this approach. Methods: Data were searched through literature, and classified discussion was conducted on this basis. The experimental group and the control group were treated in different ways, and the analysis was carried out by way of comparison, so as to explore the therapeutic effect of drug administration route on advanced ovarian cancer patients. Results: After the completion of the statistical survey, the experimental group was compared with the control group by intravenous infusion after using the new administration method. In terms of the results, the intraperitoneal infusion method had a better effect on the treatment of patients. In terms of the data, this method could effectively improve the long-term survival rate of patients. However, in terms of hepatorenal and peripheral neurotoxicity, intraperitoneal perfusion was more toxic. In terms of musculoarthralgia, the type of intraperitoneal perfusion, has a much greater effect on the patient. Conclusions: Intraperitoneal perfusion has positive therapeutic effects in patients with advanced ovarian cancer, but it is more burdsome to the body. Therefore, in the process of use, we should make comprehensive selection according to the specific situation of patients.

Open Access

Original Research Articles

Article ID: 2111

Study on the bacteriostatic effect of Baitouweng on Pseudomonas aeruginosa infection of wounds in rats

by Zhiwei Zhao, Xiaoling Li, Zhuqing Zha, Bo Cui, Yanfeng Li

Preventive Medicine Research, Vol.10, No.1, 2021; 55 Views,

Objective: To analyze the bacteriostatic effect of Baitouweng on Pseudomonas aeruginosa infection of wounds in rats. Methods: Forty Wistar rats were enrolled in the study, among which excisions were made on 30 rats on their upper layer of dorsal skin with an area of 1 cm x 1 cm, the other 10 rats as the control group of sterile wound. Prepared Pseudomonas aeruginosa was applied on the wounds of rats to create infection models. Forty rats were divided into three groups (control group, mafenide group and Baitouweng group) according to different infection methods, and were treated with normal saline, 100g/L mafenide, and 1g/L Baitouweng respectively after 3 hours of injury. The changes in the number of white blood cells in both the wound surface and body of the three groups were observed within one to four days after injury. After that, the changes on the number of both white blood cells and body weight were continuously observed. The survival of the rats in each group was observed on the 14th day after injury. Results: From the observation after injury, compared with the other two groups, rats in control group had more exudation and moist wounds, and the activities of rats decreased while the death rate increased. On the 3rd day after injury, the number of white blood cells in each group decreased, and the number of Pseudomonas aeruginosa in the control group was significantly higher than that in the other two groups (P < 0.01). The rats in the sterile wound control group did not die and continued to gain weight. After 14 days, the survival number of rats in control group was significantly less than that in mafenide group and Baitouweng group (P<0.05). Conclusion: Baitouweng has obvious bacteriostatic and virus-killing effects on Pseudomonas aeruginosa infection of wounds in Wistar rats, reducing mortality rate effectively, and has practical value as well as development and application prospects.

Open Access

Original Research Articles

Article ID: 2243

A Study on the Effect of IL-17A on Phenotypic Transformation of Fibroblasts in Bleomycin-induced Pulmonary Fibrosis in Mice and Its Mechanism

by Shuqin Ding, Xiaoyun Zhao, Yuechao Zou

Preventive Medicine Research, Vol.10, No.1, 2021; 74 Views, 8 PDF Downloads

Objective: In this study, lung fibroblasts were cultured and identified in mice lung fiber model with bleomycin. Under the induction of IL-17A, lung fibroblasts were gradually transformed into myofibroblasts in pulmonary fibrosis, and the specific induction effect of IL-17A in pulmonary fibrosis was analyzed, which could provide ideas for the prevention and treatment of clinical pulmonary fibrosis. Methods: To investigate the transcriptional expression of bleomycin-induced fractional pulmonary fibrosis in different pulmonary fibrosis processes. The 14-day mice model was taken as the research object, and the pulmonary fibrosis model was established by induction of myogenesis. After 14 days of modeling, lung tissue was removed, and after centrifugation and repeated adherent treatment, lung fibroblasts could be cultured at the origin. After three generations of culture, the morphological changes of lung fibroblasts could be observed under a microscope. Indirect immunofluorescence was used to establish the expression of vimentin, and IL-17 was used to stimulate primary cultured lung fibroblasts to detect the expression and specific localization of a-SMA in cells. Western blotting was used to stimulate the expression of lung fibroblast protein by IL-17A at different time points. Results: The typical characteristics of primary culture lung fibroblasts were obtained. After purification and culture, lung fibroblasts were obtained in morphology. The morphology of the 3rd and 4th generation cells was relatively uniform, showing long carboxyform. 1-2 nucleoli can be observed by microscope, which have distinct cell boundary and are lined up like fish schools. The results of indirect immunofluorescence showed that the vimentin staining in the third generation cells was positive, and the plasma was dark red. There were collagenous fibrous septa between the cells, which might make them develop into lung fibroblasts. A-SMA immunofluorescence results showed that in the absence of IL-17A induction, A-SMA signal was relatively weak in the lung fibroblasts of the control group and was in the cytoplasm, while after IL-17A induction, A-SMA signal was stronger in the lung fibroblasts of mice and the whole cells presented spindle structure. Western bletting showed that lung fibroblasts were stimulated by IL-17 in the 0h group. Compared with the 1h, 2h, and 4h groups, the expression of A-SMA in lung fibroblasts was significantly increased in the 1h, 2h, and 4h groups. The fibroblasts were very low in the 2h and 4h groups. There was no significant difference in the expression of AS MA signal. Compared with 0h, protein contents of p-IKB-a and p-p65 were higher in lung fibroblasts at 1h, 2h and 4h. Protein expressions of Acti, 1P6, IKB-a and P65 were different in lung fibroblasts, but there was no significant difference. However, there was no significant statistical difference in the expression of these proteins in lung fibroblasts at different times. Conclusion: By differential centrifugation and repeated adhesion, bleomycin-induced lung fibroblasts can be isolated and purified, and more cell production can be obtained. The staining vimentin was strongly positive after identification by indirect immunofluorescence. The stimulation of IL-17A could gradually transform non-fibroblasts into myofibroblasts and play an important role in pulmonary fibrosis. Therefore, through experimental studies, it was found that IL-17A stimulated F-kB signal and then increased the expression of P-IKB-a and P-P65 proteins, and transformed non-phosphorylated proteins into phosphorylated proteins, thus transforming lung fibroblasts into myofibroblasts and playing a role in pulmonary fibrosis.

Open Access

Original Research Articles

Article ID: 2241

640nm red light irradiation promotes transforming growth factor β induced collagen synthesis by MAPK cell pathway in human dermal fibroblasts

by Bin Yi, Qingxia Guo, Jun He, Kejun Han, Nan Song, Jing Fan

Preventive Medicine Research, Vol.10, No.1, 2021; 55 Views, 8 PDF Downloads

Purpose Transforming growth factor β is a key regulatory factor of collagen expression in human fibroblasts. 640nm red light can regulate the proliferation and transforming growth factor β expression of fibroblasts. Mitogen-activated protein kinase signaling pathway is involved in many physiological processes, such as cell proliferation, differentiation, and apoptosis. The study aims to investigate the effect of 640nm red light irradiation on collagen expression in fibroblasts and the specific regulatory mechanism of mitogen-activated protein kinase signaling pathway. Methods The cells were treated with 640 nm red light for different irradiation time. The effect of real-time fluorescence quantitative PCR detected gene expression of transforming growth factor β and collagen. The specific regulation mechanism of mitogen-activated protein kinase signaling pathway was assessed by western blotting. Results Red light irradiation at 640nm significantly up-regulated mRNA expression of transforming growth factor β, which promoted the expression of collagen mRNA. 640nm red light activated the protein phosphorylation of ERK and inhibited the phosphorylation level of P38 in mitogen-activated protein kinase signaling pathway, which promoting the proliferation of fibroblasts and the gene expression of collagen. Conclusion : 640nm red light irradiation can promote collagen synthesis through cell proliferation and the expression of transforming growth factor β and collagen. Phosphorylation of ERK and P38 promoted cell proliferation and up-regulates mRNA level of collagen.

Open Access

Original Research Articles

Article ID: 2242

Under COVID-19 Stress, What We Have Done, How We Are Doing, and What Shall We Do?

by Shun Huang, Yang Peng

Preventive Medicine Research, Vol.10, No.1, 2021; 51 Views, 8 PDF Downloads

From the end of 2019 up to now, novel coronavirus (COVID-19) has swept the globe, bearing down menacingly, and generating massive burden not only on government and hospital, but also on almost every single person, especially medical workers. We are anesthesiologists, surgeons, and nurses in Beijing Hospital, witnesses of this cruel battle. I’d like to describe what happened in our real life in the past few months, how dose the epidemic situation change our lives and work, and what will we do in the future.