640nm red light irradiation promotes transforming growth factor β induced collagen synthesis by MAPK cell pathway in human dermal fibroblasts
Abstract
Purpose Transforming growth factor β is a key regulatory factor of collagen expression in human fibroblasts. 640nm red light can regulate the proliferation and transforming growth factor β expression of fibroblasts. Mitogen-activated protein kinase signaling pathway is involved in many physiological processes, such as cell proliferation, differentiation, and apoptosis. The study aims to investigate the effect of 640nm red light irradiation on collagen expression in fibroblasts and the specific regulatory mechanism of mitogen-activated protein kinase signaling pathway. Methods The cells were treated with 640 nm red light for different irradiation time. The effect of real-time fluorescence quantitative PCR detected gene expression of transforming growth factor β and collagen. The specific regulation mechanism of mitogen-activated protein kinase signaling pathway was assessed by western blotting. Results Red light irradiation at 640nm significantly up-regulated mRNA expression of transforming growth factor β, which promoted the expression of collagen mRNA. 640nm red light activated the protein phosphorylation of ERK and inhibited the phosphorylation level of P38 in mitogen-activated protein kinase signaling pathway, which promoting the proliferation of fibroblasts and the gene expression of collagen. Conclusion: 640nm red light irradiation can promote collagen synthesis through cell proliferation and the expression of transforming growth factor β and collagen. Phosphorylation of ERK and P38 promoted cell proliferation and up-regulates mRNA level of collagen.
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Copyright (c) 2021 Bin Yi, Qingxia Guo, Jun He, Kejun Han, Nan Song, Jing Fan
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