Study on the bacteriostatic effect of Baitouweng on Pseudomonas aeruginosa infection of wounds in rats

Zhiwei Zhao; Xiaoling Li; Zhuqing Zha; Bo Cui; Yanfeng Li

doi:10.18282/pmr.v10i1.2111

Zhiwei Zhao Department of Hand Microsurgery, Luoyang Orthopedic-Traumatological Hospital of Henan Province
Xiaoling Li Health Management Center, Luoyang Orthopedic-Traumatological Hospital of Henan Province
Zhuqing Zha Department of Hand Microsurgery, Luoyang Orthopedic-Traumatological Hospital of Henan Province
Bo Cui Department of Hand Microsurgery, Luoyang Orthopedic-Traumatological Hospital of Henan Province
Yanfeng Li Department of Hand Microsurgery, Luoyang Orthopedic-Traumatological Hospital of Henan Province

Ariticle ID: 2111

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DOI: https://doi.org/10.18282/pmr.v10i1.2111

Keywords: Baitouweng, Pseudomonas Aeruginosa, Wound Infection

Abstract

Objective: To analyze the bacteriostatic effect of Baitouweng on Pseudomonas aeruginosa infection of wounds in rats. Methods: Forty Wistar rats were enrolled in the study, among which excisions were made on 30 rats on their upper layer of dorsal skin with an area of 1 cm x 1 cm, the other 10 rats as the control group of sterile wound. Prepared Pseudomonas aeruginosa was applied on the wounds of rats to create infection models. Forty rats were divided into three groups (control group, mafenide group and Baitouweng group) according to different infection methods, and were treated with normal saline, 100g/L mafenide, and 1g/L Baitouweng respectively after 3 hours of injury. The changes in the number of white blood cells in both the wound surface and body of the three groups were observed within one to four days after injury. After that, the changes on the number of both white blood cells and body weight were continuously observed. The survival of the rats in each group was observed on the 14th day after injury. Results: From the observation after injury, compared with the other two groups, rats in control group had more exudation and moist wounds, and the activities of rats decreased while the death rate increased. On the 3rd day after injury, the number of white blood cells in each group decreased, and the number of Pseudomonas aeruginosa in the control group was significantly higher than that in the other two groups (P < 0.01). The rats in the sterile wound control group did not die and continued to gain weight. After 14 days, the survival number of rats in control group was significantly less than that in mafenide group and Baitouweng group (P<0.05). Conclusion: Baitouweng has obvious bacteriostatic and virus-killing effects on Pseudomonas aeruginosa infection of wounds in Wistar rats, reducing mortality rate effectively, and has practical value as well as development and application prospects.

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